This purified polyclonal antibody reacts with human SUMO by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions. For immunoblotting a 1:2,000 dilution is recommended. An 11.6 kDa band corresponding to human SUMO is detected. Most human cell lysates can be used as a positive control without induction or stimulation. For ELISA a 1:4,000 to 1:20,000 dilution is recommended. Researchers should determine optimal titers for other applications.