Anti-PDL-1 Antibody has been tested in Western Blot, ELISA, and IF. Positive control whole cell lysates used MDA-MB-231 and A549 and positive recombinant protein control for western blotting. Expect a band at ~33.3 kDa in western blot using appropriate lysates. Immunofluorescence positive control cell line used was MDA-MB-231. Although not tested, this antibody is likely functional in flow cytometry and immunoprecipitation.