This Protein G purified antibody has been tested for use in ELISA, immunoprecipitation, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Approximately 0.7 µg of antibody is sufficient to detect 5 µg of SIN-1 treated BSA by Western blotting using HRP Gt-a-Mouse IgG and chemiluminescent substrate.