This antibody has been tested for use in ELISA and western blot. A 1:500 dilution was effective for staining FFPE human kidney tissue by Immunohistochemistry (IHC). Specific conditions for reactivity should be optimized by the end user. For western blot experiments, one can expect a band of ˜110 kDa in size corresponding to active Notch 2 in the appropriate cell lysate.