Anti-HA-Peroxidase, High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10), conjugated to peroxidase.
Immunogen
The epitope consists of amino acids 98-106 from the human influenza virus hemagglutinin protein.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Preparation Note
Stabilizers: proteinaceous stabilizers
Working concentration: The working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
- Dot blot: 50 mU/ml
- ELISA: 25 mU/ml
- Western blot: 50 mU/ml
Reconstitution
- Add 1.0 ml double-distilled water for a final concentration of 25 U/mL
- Rehydrate for 10 min prior to use
Specificity
Anti-HA-Peroxidase, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein.
This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
Application
- Use Anti-HA-Peroxidase, High Affinity for the detection of HA-tagged recombinant proteins using: ELISA
- Western blot[1][2]
Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.
[1] The protein synthesis inhibitor blasticidin s enters mammalian cells via leucine-rich repeat-containing protein 8D.
[2] Monoubiquitination of filamin b regulates vascular endothelial growth factor-mediated trafficking of histone deacetylase 7