Synonyms: BSA; Bovine albumin
CAS No.: 9048-46-8
Storage Temperature: 2-8°C
Application: A non-acetylated protein suitable as a blocking agent in Southern blotting. If your application requires nuclease-free BSA, we recommend our acetylated BSA for these critical applications.
Biochem/physiol Actions: Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.1
Preparation Note: Serum albumin may be referred to as Cohn Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Cohn Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Protocols & Applications: Select the right albumin for your application with the Albumin Selection Guide
Foreign Activity: protease, none detected
Form: lyophilized powder
Grade: for molecular biology
Mol Wt: mol wt ~66 kDa
Product Line: BioReagent
Storage Temp.: 2-8°C