The total protein is determined using the Biuret procedure, with bovine albumin as the standard. Each vial is overfilled by approximately 10 percent so that a minimum of 50 mg of mouse albumin Fraction V is obtained upon reconstitution.
Mouse albumin Fraction V is purified from pooled mouse serum using multi-step procedures which may include salt fractionation, gel filtration and ion-exchange chromatography. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative added.
Albumin, Mouse can be used for making immune complexes with anti-albumin antibodies. Fatty acids and globulins will not interfere with the complex formation.
Product is the lyophilized powder of mouse albumin Fraction V and buffer salts.
Assay: The product is tested for purity at a minimum of 20 mg/mL using immunoelectrophoresis. The product is mouse albumin Fraction V; no trace of immunoglobulins is detected.
Concentration: Total Protein: 16.8 mg/mL; Volume: 3.0 mL
Key Applications: Immune complexes
Application Areas: Immunoassays
Product Type: Reagents
Format: Lyophilized
NOTES: Purified Fraction V
Solubility: Reconstitution: Reconstitute product with 3.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Storage & Handling: Store unopened product at 2-8°C. Store reconstituted material no longer than a few days at 2-8°C unless sodium azide is added. For long-term storage, aliquot and freeze at -20°C.