Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Transferrin is involved in iron transport to developing red cells for hemoglobin synthesis. Each protein molecule specifically binds with two Fe3+ ions to form salmon-pink complexes; bicarbonate or carbonate ions are involved in the formation of these colored complexes.
Bovine Transferrin (HOLO) is frequently used as a nutrient in cell and microbial culture and as a media supplement in bio-pharmaceutical production. Bovine holo-transferrin can be used with a wide range of cell types.
A group of homologous non-heme, iron-binding glycoproteins. It is an important factor in culture work to carry metabolites to the cells, particularly in a defined medium. Composed of two homologous domains, each containing a binding site for metal ions. The sites are similar, but not identical, in their metal-binding properties.
Bovine Transferrin is chromatographically purified from New Zealand-sourced bovine plasma in an ISO quality system assuring complete traceability and consistent high quality. Transferrin (HOLO) is an iron-rich product supplied as a heat-treated, lyophilised powder.
Purity: ≥95% Transferrin w/w protein
Concentration: Total Protein (anhydrous) ≥95% w/w total protein
Key Applications: Media supplement in bio-pharmaceutical production
Application Areas: Cell Culture
Research Areas: Embryonic Stem Cells; Neural Stem Cells
Product Type: Proteins, Enzymes & Peptides
Presentation: Red to Brown Powder
pH: 6.0 - 8.0 (3% w/v soln)
Foreign activity: Mycoplasma None Detected; Virus (9 CFR113.53(c)) None Detected
Moisture content: ≤5.0% w/w
NOTES: Iron ≥1200 µg/g protein; Bioburden ≤100 cfu/g; Endotoxin ≤1 EU/mg
Solubility: Soluble in water (50 mg/mL - clear, dark orange-red solution); (1% aq soln) Dissolves completely in 20 minutes at 20 - 25°C.
Storage & Handling: Store at +4°C.