General description: Modification of the original MEM formulation. Earle's salts have been replaced with Hanks' salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity. It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.
Quantity: Formulated to contain 10.7 grams of powder per liter of medium.
Reconstitution: Supplement with 0.35 g/L sodium bicarbonate.
Empirical Formula (Hill Notation): C6H5F