Description: : Purified, Staphylococcus aureus Panton-Valentine Leukocidin (PVL) F subunit (LukF-PV). The theoretical molecular weight of the protein is 35,580 Daltons
Size: 100 ug of protein is supplied in PBS at a concentration of 2.994 mg/mL. Protein demonstrates a molecular weight of approximately 37 kDa
Relevance: This protein may be used in functional PVL toxicity assays in combination with LukS-PV, or as a control protein in ELISA assays or Western blotting when detecting LukF in PVL (+) strains of S. aureus
Recommended Dilutions:
ELISA: Assay-dependent dilution
WB: Assay-dependent dilution; internal QC demonstrates detection of 100 ng of LukF-PV protein when detected with antiPVL LukF polyclonal antibody (cat: 0312-002) in Western blotting
PVL Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with LukS-PV in a concentration range of 1-100 nM.
Storage: 2-3 weeks at -20°C, -80°C long term.
Intended for research use only, not for human, therapeutic, or diagnostic applications.