IL-2, Interleukin-2, murine (mouse): Murine Interleukin-2
Mature mouse IL-2 shares 56% and 73% aa sequence identity with human and rat IL-2, respectively. It shows strain-specific heterogeneity in an N-terminal region that contains a poly-glutamine stretch. Mouse and human IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. The 55 kDa IL-2 Rαis specific for IL-2 and binds with low affinity. The 75 kDa IL-2 Rβ, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chainγc/IL-2 Rγ, which is shared with the receptors for IL-4, -7, -9, - 15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 Rβandγc. It drives resting T cells to proliferate and induces IL-2 and IL-2 Rαsynthesis. It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes. IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells.
Source: Escherichia coli.
Molecular Weight: Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 149 amino acids.
Quantity: 5ug/20ug/1000µg
Purity: >95% by SDS-PAGE and HPLC analyses.
Biological Activity: The ED50 as determined by the dose dependent stimulation of murine CTLL-2 cells is < 0.2 ng/ml, corresponding to a specific activity of > 5 x 106 units/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.
AA Sequence: PTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDLQELLSR MENYRNLKLP RMLTFKFYLP KQATELKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ FDDESATVVD FLRRWIAFCQ SIISTSPQ
Endotoxin: Less than 1EU/mg of rmIL-2 as determined by LAL method.