Rabbit antiserum to rat albumin is the lyophilized powder of rabbit antiserum to rat albumin and buffer salts.
Rabbit antiserum to rat albumin product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. Note: IgG fractions are suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers and for attachment to solid supports for use as an immunoadsorbant.
RECONSTITUTION: Reconstitute product with 1.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
The antiserum is prepared by hyperimmunizing rabbits with highly purified rat albumin; antiserum is delipidated and made specific by solid-phase absorption. The resulting antiserum is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized by equivalence-point precipitation. Each antiserum is tested for specificity at a minimum of 80 mg/mL using immunoelectrophoresis.