SPINeasy Plasmid Miniprep Kit is a silica membrane spin column kit that enables the purification of up to 20 µg of plasmid DNA from 1 – 5 mL of transformed bacterial cultures. High-purity plasmid DNA can be obtained within 25 minutes through a simple purification process, immediately ready for routine molecular biology applications including cloning, restriction digestion, PCR, and in-vitro transcription.
Application Notes:
The SPINeasy Plasmid Miniprep Kit quickly and efficiently purifies up to 20 µg plasmid DNA from 1-5 mL of transformed bacterial culture within 25 minutes through a simple purification process.
Usage Statement:
Research Use Only (RUO)
Key Applications:
In-vitro transcription, cloning, NGS, PCR, qPCR, restriction digestion, transformation, Genomics, Molecular Biology
Key features and details
- Spin column technology for fast, high-quality plasmid DNA isolation from 1-5 mL of bacterial cultures
- High purity plasmid DNA, free of gDNA contamination following a modified alkaline lysis method
- Plasmid DNA suitable for a variety of downstream applications, including cloning, restriction digestion, and transformation
Alternate Names: SPINeasy Plasmid Extraction Kit; Plasmid Miniprep Kit; Plasmid DNA Purification
Application Notes: The SPINeasy Plasmid Miniprep Kit quickly and efficiently purifies up to 20 µg plasmid DNA from 1-5 mL of transformed bacterial culture within 25 minutes through a simple purification process.
Base Catalog Number: 65340
Brand: SPINeasy
Components: 20 mL Resuspension Buffer SN1, 20 mL Alkaline Lysis Buffer SN2, 30 mL Neutralization Buffer SN3, 21 mL Wash Buffer NW1, 6 mL Wash Buffer NW2, 10 mL Elution Buffer SN, 200 µL RNase A, 50x Column SN with collection tubes
Hazard Statements: Hazard Class 8
Pack Size: 50 Preps
Personal Protective Equipment: Safety Goggles/Faceshield; Gloves
Storage: Store at Room Temperature (15-30?), Dry environment
Usage Statement: Research Use Only (RUO).