SPINeasy DNA Kit for Blood is a high-performance genomic DNA (gDNA) extraction kit based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from whole blood preserved in different anticoagulants (EDTA, heparin, and sodium citrate), typically in less than 30 minutes. It also allows easy gDNA isolation from coagulated blood when using the FastPrep-24 5G homogenizer with lysing matrix A or D tubes (recommended for soft tissues, and not included with this kit). The use of our specially formulated Lysis Buffer BL enables highly efficient lysis of various liquid samples including plasma, serum, saliva, and cell culture medium. Column S1 and kit buffers (provided in the kit) are designed to deliver gDNA extracts of high yield and purity, compatible with downstream molecular applications such as PCR and NGS.
Application Notes:
For the extraction of high quality gDNA from a variety of sample sources, including fresh, frozen, and anticoagulated blood; plasma; serum; and cell culture medium for downstream applications such as restriction digestion, PCR and NGS analysis.
Usage Statement:
Research Use Only (RUO)
Key Applications:
Genomics; Molecular Biology; PCR, qPCR, Microbiome analysis, NGS; shotgun sequencing
Key features and details
- Fast and efficient DNA isolation from blood and other biofluids in less than 30 minutes
- Convenient spin-column technology with no organic solvents
- High DNA yield and purity, free of common contaminants
- DNA ready for downstream PCR or NGS applications
Alternate Names: Spin-column DNA Extraction Kit from Blood, gDNA Extraction kit for blood
Application Notes: For the extraction of high quality gDNA from a variety of sample sources, including fresh, frozen, and anticoagulated blood; plasma; serum; and cell culture medium for downstream applications such as restriction digestion, PCR and NGS analysis.
Base Catalog Number: 52050
Brand: SPINeasy
Components: Lysis Buffer BL, Wash Buffer BW1, Wash Buffer BW2, Elution Buffer EB, Column S, 2.0 mL collection tubes,1.5 mL microcentrifuge tubes, Proteinase K, Proteinase K Buffer
Pack Size: 50 Preps
Personal Protective Equipment: Gloves
Sample Volume: 200 μL of Human blood, serum, plasma, saliva, or cell culture media
Storage: 15-25 ?
Usage Statement: Research Use Only (RUO)