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Zymo Research Corporation

Zymo-Seq RRBS Library Ki

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Zymo-Seq RRBS Library Kit is an all-in-one RRBS library prep kit that generates NGS libraries with a simplified workflow for the classic method Reduced Representation Bisulfite Sequencing (RRBS). This all-in-one RRBS library prep kit allows for DNA methylation profiling at single-nucleotide resolution in CpG-rich regions of the genome. This Zymo-Seq RRBS Library Kit is the ideal RRBS library prep kit to generate libraries that cover a significant percent (=70% if using human genomic DNA) of all CpG islands, promotors, and gene bodies while largely reduces the sequencing cost, making it ideal for high-throughput screening and comparison experiments. Now includes Unique Dual Index (UDI) Primer Sets.

Equipment RequiredMicrocentrifuge, thermocycler with a heated lid
Input10 – 500 ng of genomic DNA
Sample SourcesPurified genomic DNA. The DNA should be free of enzymatic inhibitors and can be suspended in water, TE, or a low salt buffer. DNA with low 260/280 or 260/230 ratios should be purified prior to processing using the Genomic DNA Clean & Concentrator (Cat. No. D4010). This protocol is not recommended for samples from species with low CpG density.
Sequencing Platform CompatibleLibraries are compatible with all Illumina sequencing platforms except HiSeq X Series.

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Product Detail
Thomas No.
CHM00B727
Mfr. No.
D5460
Description
Zymo-Seq RRBS Library Kit (24 preps)
UN Number
UN1845
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product CHM00B727 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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