This kit and procedure will allow the the preparation of both a cytoplasmic fraction and a nuclear extract from mammalian cells or tissue.
Contains reagents for 250 preps of 20uL packed cell pellets or 50 preparations of 100uL cell pellets (~10g cell paste total).
Kit Contents
- 40mL Cytoplasmic Extraction Buffer (CEB)
- 3mL Nuclear Extraction Buffer (NEB)
- 300µL Extraction Buffer Additive (EBA) – 200X solution
- 100mL 1X PBS (pH 7.2)