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qTOWER 2.0/2.2 Quantitative Real-Time PCR Thermal Cycler

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qTOWER 2.0/2.2 Quantitative Real-Time PCR Thermal Cycler

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qTOWER 2.0/2.2 models offer quantitative real-time PCR with proven 96 well standard SBS format. Top-reading fluorescence detection design has patented high performance optical components with color and FRET modules for excitation and emission.

Systems feature state-of-the-art heating rates up to 5.5°C/sec and cooling rates of 4.0°C/sec. High-speed scan capability as fast as 6 sec. for a 96 well microplate. Flexible use of different optical plastic materials: 0.2ml tubes, 8 well strips or 96 well microplates.

qTOWER 2.2 offers gradient function across 12 columns with a max. temperature range of 40°C

Select from interchangeable color and FRET modules (ordered separately) to meet specific wavelength requirements. Modules are placed in up to 6 positions. Depending on the application, it can be adapted to either intercalating dyes, hydrolysis probes or hybridization probes (FRET probes).

The thermal block is made of gold-coated silver to achieve the best possible performance and maximum thermal conductivity resulting outstanding homogeneity and uniformity of temperature.

qPCRsoft quantitative analysis software (MIQE compliant) which loads on your PC. The program can determine PCR efficiencies and perform aboslute and relative quantifications as well as delta-delta Ct method and allele discrimination.

10 years on optics components; 2 years on the system and thermal block.

Optical system
Principle of measurementTop-reading fluorescence detection via 8 optical fibers with color modules for excitation and emission filters
Light sourceHigh-power, long-life LEDs
Detector
  • CPM - channel photo multiplier
  • Highly sensitive
  • Increased SNR
Number of color modules
  • 12 available
  • 6 positions inside device
Analytical parameters
Sensitivity1 nM FAM™ in minimal 30 µl sample volume
Read-out time6 seconds for 96 wells independent of the number of dyes to be measured
Microplate format96 wells for 96 well microplates, 
8 well strips or individual tubes
Sample volume10 – 60 µl
System and application parameters of the thermal cycler
Heating rate5.5°C/sec max
Cooling rate4.0°C/sec max.
Block homogeneity± 0.2°C
Control accuracy± 0.1°C
Sample block temperature3°C - 99°C
Time incr./decr.± 0.1 to 1 sec/cycle
Temperature incr./decr.± 0.1 to 1°C/cycle
Contact pressure10 kg/plate, automatically
No. of programsNot limited
GradientMax. 40°C across 12 columns
Lid
  • Heated lid up to 110 °C
  • SPS technology
Parameters: Color Modules
NameExcitationEmissionDyes (examples)*
Color module 1470 nm520 nmFAM™, Sybr®Green, Alexa488®
Color module 2515 nm545 nmJOE™, HEX™, VIC®, YakimaYellow™
Color module 3535 nm580 nmTAMRA™, DFO™, Alexa546®, NED™
Color module 4565 nm605 nmROX™, TexasRed®, Cy3.5®
Color module 5630 nm670 nmCy5®, Alexa633®, Quasar670™
Color module 6660 nm705 nmCy5.5®, LightCycler Red®
FRET 1470 nm580 nmFAM™ (donor) / TAMRA™ (acceptor)
FRET 2470 nm670 nmFAM™ (donor) / Cy5® (acceptor)
FRET 3470 nm705 nmFAM™ (donor) / Cy5.5® (acceptor)
FRET 4515 nm670 nmJOE™ (donor) / Cy5® (acceptor)
FRET 5470 nm605 nmFAM™ (Donor) / ROX™ (acceptor)
Color module Protein 1490 nm580 nmSYPRO® Orange
* Color or FRET modules can be ordered separately. The qTOWER 2.0 or 2.2 can be equipped with up to 6 modules.

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Product Detail
Thomas No.
1185D70
Mfr. No.
844-00520-0
Description
Color Module 1 for use with a variety of dyes including FAM™, Sybr® Green, Alexa488®
list price/quantitytotal
$0.00
Thomas No.
1185D71
Mfr. No.
844-00521-0
Description
Color Module 2 for use with a variety of dyes including JOE™, HEX™, VIC®, YakimaYellow®
list price/quantitytotal
$0.00
Thomas No.
1185D72
Mfr. No.
844-00522-0
Description
Color Module 3 for use with a variety of dyes including TAMRA™, DFO™, Alexa546®, NED™
list price/quantitytotal
$0.00
Thomas No.
1185D73
Mfr. No.
844-00523-0
Description
Color Module 4 for use with a variety of dyes including ROX™, TexasRed®, Cy3.5®
list price/quantitytotal
$0.00
Thomas No.
1185D74
Mfr. No.
844-00524-0
Description
Color Module 5 for use with a variety of dyes including Cy5®, Alexa633®, Quasar670™
list price/quantitytotal
$0.00
Thomas No.
1185D75
Mfr. No.
844-00525-0
Description
Color Module 6 for use with a variety of dyes including Cy5.5®, LightCycler Red®
list price/quantitytotal
$0.00
Thomas No.
1185D76
Mfr. No.
844-00526-0
Description
FRET 1 for use with a variety of dyes including FAM™ (Donor) / TAMRA™ (acceptor)
list price/quantitytotal
$0.00
Thomas No.
1185D77
Mfr. No.
844-00527-0
Description
FRET 2 for use with a variety of dyes including FAM™ (Donor) / Cy5® (acceptor)
list price/quantitytotal
$0.00
Thomas No.
1185D78
Mfr. No.
844-00528-0
Description
FRET 3 for use with a variety of dyes including FAM™ (Donor) / Cy5.5® (acceptor)
list price/quantitytotal
$0.00
Thomas No.
1185D79
Mfr. No.
844-00529-0
Description
FRET 4 for use with a variety of dyes including JOE™ (Donor) / Cy5® (acceptor)
list price/quantitytotal
$0.00
Thomas No.
1185D80
Mfr. No.
844-00531-0
Description
FRET 5 for use with a variety of dyes including FAM™ (Donor) / ROX™ (acceptor)
list price/quantitytotal
$0.00
Thomas No.
1185D81
Mfr. No.
844-00530-0
Description
Color Module Protein 1 for use with a variety of dyes including SYPRO® Orange
list price/quantitytotal
$0.00
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