Tissue Culture Media
The primary purpose of tissue culture media is to provide a synthetic environment within the lab. This synthetic environment then allows cells to thrive and carry out normal functions.
Ideal for primary cell isolation and cell line maintenance, culture media is essential for the isolation and propagation of primary cells obtained from tissues.
PBS cell culture media is recommended for use when investigating the localization and distribution of target proteins in different parts of biological tissue.
Tissue culture media accessories include reagent-grade water, antibiotics, and Corning BioCoat cell culture inserts to assist you with achieving clear and accurate results.
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Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of…
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This 1:1 mixture of Dulbecco's Modified Eagle Medium and Ham's F-12 media is ideal for culture of a variety of cell types under low serum conditions. Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the…
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Minimum Essential Medium (MEM)
CorningMinimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells. This research indicated that additions made to BME aided cell propagation. MEM is modified with higher…
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EMEM with L-Glutamine
Quality BiologicalEagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations. Applications MEM was developed by Dr. Harry Eagle to overcome problems encountered when attempting to grow…
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This Tryptic Soy Broth (TSB) product is offered for use in sterility testing and validation in the manufacture of a liquid product. The use of this medium in place of the product intended for production allows an accurate assessment of sterility throughout the manufacture process. The product is a…
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Catalase Reagent Droppers are used in a qualitative procedure for determining catalase activity by bacteria. The enzyme catalase is preset in most aerobic and facultatively anaerobic bacteria, with the major exception of streptococci and enterococci. The enzyme catalase decomposes hydrogen…
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Poly-D-Lysine, 20 mg
CorningPoly-D-Lysine, used to culture a wide variety of cell types, particularly neurons, glial cells, and transfected cells. (20 mg) Source: Synthetic Quality: Shown to promote cell attachment and spreading of rat cerebellar granule cells Filtered (0.2 µm membrane) and tested and found negative…
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CMRL media are based on the composition of Medium 199. Nucleic acid derivatives, coenzymes omitted in H597 modification were retained in CMRL 1066. CMRL 1066 is a chemically defined medium formulated for long term cultivation of mouse L cells in the absence of protein supplement. Although…
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Corning transfectagro Reduced Serum Medium is designed to maximize the growth of a variety of cell types under reduced serum conditions, generally allowing for at least a 50% reduction in serum use with minimal adaptation. transfectagro has also been proven to improve transient transfection…
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A modification of Ham's F-12, F-12K contains higher concentrations of amino acids, sodium pyruvate and other components than the original. It is formulated to support the growth of cells including differentiated rat and chicken cell types and primary human hepatocytes. This medium contains…
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Collagen I, found in most tissues and organs, is most plentiful in dermis, tendon, and bone. It is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture. in vitro use of collagen can exert effects on the…
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Buffers
CorningSodium bicarbonate Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7.2 - 7.4 in a culture environment. Bicarbonate is a weak buffer with that makes maintaining a pH range of 7.2 - 7.6 somewhat difficult, but it is non-toxic, and is a…
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Type IV Collagen is a ubiquitous component in basement membranes and provides the major structural support for this matrix. When the Collagen IV meshwork is assembled, it provides a scaffold for the assembly of other basement membrane components through interactions with laminin, entactin/nidogen,…
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Type IV Collagen is a ubiquitous component in basement membranes and provides the major structural support for this matrix. When the Collagen IV meshwork is assembled, it provides a scaffold for the assembly of other basement membrane components through interactions with laminin, entactin/nidogen,…
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RPMI-1640 without Phenol Red & L-Glutamine
Quality BiologicalRPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate Without HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial…
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High Sucrose Brain Slice Cutting Solution (HS-BSCS)
Ecocyte BioscienceHigh Sucrose Brain Slice Cutting Solution (HS-BSCS) is used for cutting acute brain slices from rats and mice. Product information Ready to use media autoclaved Use immediately once opened. Store at 4 - 8 °C Ional composition of HS-BSCS Solution (Base A + Base B) Sucrose =…
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Cefinase™ discs are intended for use in rapid testing of isolated colonies of Neisseria gonorrhoeae, Staphylococcus species, Haemophilus influenzae, enterococci and anaerobic bacteria for the production of β-lactamase.
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Corning® BioCoat™ Gelatin Flasks
CorningCorning BioCoat Gelatin cultureware provides an attachment and growth promoting substrate for the culture of a variety of cell types. Gelatin is commonly used in the culture of vascular endothelial cells, muscle, embryonic stem (ES) cells, and F9 teratocarcinoma cells. It is also suitable for…
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Leibovitz' L-15 Medium without L-Glutamine
Quality BiologicalDesigned for cell culture without CO2. Without Sodium Bicarbonate With Phenol Red Without L-glutamine Applications L-15 has been used for the culture of HEp-2 monkey kidney and primary explants of embryonic and adult human tissue, and a variety of other cells. Many viruses have also been…
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Leibovitz's L-15
CorningLeibovitz's L15 medium was originally developed without a sodium bicaronate buffer system for use in non-CO2 equilibrated environments. It is buffered by phosphates and the amino acid L-arginine, along with L-histidine and L-cysteine. The formation of acid metabolite byproducts is prevented by…
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Para-Marker
Electron Microscopy SciencesA wide tipped pen, designed for use with paraffin sections. The pen provides a sticky membrane to the glass slide which not only prevents paraffin sections from falling off but also keeps the sections flat on the slide.
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HTS insert plates are arrays of individual cell culture inserts connected by a rigid, robotics-friendly holder. This single-unit design makes insert plates ideal for running automated, high throughput drug transport (Caco-2 cells) cell toxicity studies or cell migration and invasion studies. …
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MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the…
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Sodium Pyruvate Solution 100mM
HiMediaSodium Pyruvate Solution 100mM. Used as a medium supplement for energy source and a carbon skeleton for anabolic processes.
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The Corning® BioCoat® Poly-D-Lysine coverslip and dish is perfect for enhanced cell attachment and growth of a variety of primary cells as well as transformed cells in serum-free or serum-containing cultures..
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Intended Use: Taxo™ A discs are for the presumptive identification of group A beta-hemolytic streptococci based on susceptibility to a low level of bacitracin. Discs are intended for use with pure cultures, with the exception noted under "Specimens." Taxo™ P Discs…
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Hanks' Balanced Salt Solution 1X w/ Phenol red and Sodium bicarbonate w/o Calcium. Used with cells maintained in less CO2 environment or CO2 free environment.
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Trace Element Solution 2
MP BiomedicalsMP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It has the same composition as Cleveland’s Trace Element I. To be added aseptically to media as a supplement. Prior to use of media, it may be necessary to adjust pH and sterile filter (optional, reduces contamination).