Tissue Culture Media
The primary purpose of tissue culture media is to provide a synthetic environment within the lab. This synthetic environment then allows cells to thrive and carry out normal functions.
Ideal for primary cell isolation and cell line maintenance, culture media is essential for the isolation and propagation of primary cells obtained from tissues.
PBS cell culture media is recommended for use when investigating the localization and distribution of target proteins in different parts of biological tissue.
Tissue culture media accessories include reagent-grade water, antibiotics, and Corning BioCoat cell culture inserts to assist you with achieving clear and accurate results.
-
The Corning portfolio of cytokines, growth factors, and media supplementation offers a wide array of proteins that result in proliferation or differentiation of the affected cells. Vialed, highly purified growth factors with high biological activity Broad range of highly purified natural and…
-
All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
-
Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…
-
Minimum Essential Medium (MEM)
CorningMinimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells. This research indicated that additions made to BME aided cell propagation. MEM is modified with higher…
-
Trace Elements A, B, and C
CorningTrace Elements A provides copper, zinc, iron, and selenium. Selenium aids in the detoxification of free radicals as a cofactor for GSH synthetase, while iron, copper, and zinc may be bound by serum protein. The combination of Trace Elements A contains the trace elements of MCDB 104 and of MCDB…
-
Dulbecco’s Modified Eagle Medium (DMEM) w/ 4.5gms Glucose per litre, L- Alanyl-L-Glutamine, 3.7 gms per litre Sodium bicarbonate and Sodium pyruvate. Contains stabililzed dipeptide of L-Glutamine.
-
Sodium Pyruvate Solution 100mM
HiMediaSodium Pyruvate Solution 100mM. Used as a medium supplement for energy source and a carbon skeleton for anabolic processes.
-
Collagen I, found in most tissues and organs, is most plentiful in dermis, tendon, and bone. It is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture. in vitro use of collagen can exert effects on the…
-
NanoShuttle-PL Refills
Greiner Bio-OneNanoShuttle™-PL consists of gold, iron oxide, and poly-L-lysine. NanoShuttle™-PL magnetizes cells by electrostatically attaching to cell membranes during an overnight static incubation. Magnetized cells will appear peppered with dark nanoparticles after incubation. NanoShuttle™-PL…
-
Accutase® cell detachment solution contains proprietary marine-origin enzymes with proteolytic and collagenolytic activity for the detachment of primary and stem cell lines and tissues. It’s useful for the routine detachment of cells from standard tissue culture plasticware and adhesion…
-
Prepared RODAC™ (Replicate Organism Detection and Counting) plates are recommended for the detection and enumeration of microorganisms present on surfaces of sanitary importance.
-
-
Phosphate Buffered Saline, pH 7.2 10X. Commonly used for tissue disaggregation and monolayer dispersal.
-
Amino Acids & Vitamins
CorningL-glutamin L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing toxic compounds.…
-
Dulbecco’s Modified Eagle’s Medium - High Glucose
MilliporeSigmawith 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is…
-
QBSF®-60 Serum Free Medium
Quality BiologicalQBSF-60 is a proprietary serum-free medium that supports the expansion of human hematopoietic stem/progenitor cells. QBSF-60 supports CD34+ cells derived from normal bone marrow, cord blood, peripheral blood stem cells mobilized with cytokines for ex-vivo expansion and differentiation to…
-
FlexiPump® Pro Complete Dispensing Assembly
InterscienceThe FlexiPump Pro complete dispensing assembly with a Y connector makes it easy to gain access to various liquid samples without the risk of any harmful consequences.
-
Corning® MCDB 131 1X
CorningMCDB 131 was originally developed by the researchers Knedler and Ham for the low-protein and serum free growth of human microvascular endothelial cells (HMVEC). Since its development, this medium has been used for this cell type and others such as liver, smooth muscle, cardiac myocytes. …
-
Corning insectagro Sf9 is formulated to support the propagation of Sf9 insect cells in culture, and can also be used for SF21 cells. The Sf9 cell line originated at the USDA Insect Pathology Laboratory from IPLBSF-21 cell line, derived from the pupal ovarian tissue of the fall army worm. Sf9 cells…
-
BBL™CHROMagar™ Orientation medium is a nonselective differentiated medium for the isolation, differentiation and enumeration of urinary tract pathogens. BBL CHROMagar Orientation medium allows for the differentiation and identification of Escherichia coli and Enterococcus without…
-
Interleukin-2 (IL-2), mouse recombinant, plays a central role in cell-mediated immune response. Was first identified by its ability to promote the long-term proliferation of activated T-cells. (25,000 BRMP units) References: 1. Hatakeyama, M. and Taniguchi, T., "Interleukin-2" in Peptide Growth…
-
-
RPMI 1640
CorningNamed for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and hybridoma cells such as mouse…
-
Liquid Blocker–Super PAP Pen
Electron Microscopy SciencesThe Newest Staining Guard Pen New and improved. The Super PAP Pen outperforms the original PAP Pen. The Super PAP Pen may be used at temperatures as high as 120°F. (The old style PAP Pen worked only up to 90°F). With this high temperature feature, one can now use the Super PAP Pen to…
-
Corning® glutagro™ DMEM
CorningDulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of…
-
Cryopreservation Medium with FBS
Quality BiologicalApplications The FBS in the Cryopreservation Medium improves the post-freezing recovery and survival after cryopreservation, while DMSO preserves the cells during freeze. Cryopreservation Medium with Fetal Bovine Serum (FBS) and Calf Serum is a complete cryopreservation medium with 10% DMSO.
-
HEPES is a zwitterionic organic buffering agent. It is stable, water soluble and does not react with divalent cations. It has a pKa of 7.5 at 37deg C and offers an optimal buffering range of pH 6.8 - 8.2. It maintains the physiological pH despite changes in the carbon dioxide concentrations which…
-
All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
-
Serum Free Cryo Medium
Quality BiologicalQBI's Serum Free Cryo Medium is a sterile, 2X concentrated freezing medium containing DMSO developed for the cryopreservation of cells grown under serum free conditions. Serum Free Cryo Medium is an animal-free, protein-free, chemically defined medium containing DMSO and other…
-
Hanks' Balanced Salt Solution (HBSS) without Phenol Red
Quality BiologicalHanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport…
-
TCL016 is molecular biology grade sterile filtered water, treated with 0.1% Diethylpyrocarbonate(DEPC). It is prepared by passing water through continuous process which includes water pretreatment, reverse osmosis, deionization, and filtration. It is produced specifically for molecular biology…