Tissue Culture Media
The primary purpose of tissue culture media is to provide a synthetic environment within the lab. This synthetic environment then allows cells to thrive and carry out normal functions.
Ideal for primary cell isolation and cell line maintenance, culture media is essential for the isolation and propagation of primary cells obtained from tissues.
PBS cell culture media is recommended for use when investigating the localization and distribution of target proteins in different parts of biological tissue.
Tissue culture media accessories include reagent-grade water, antibiotics, and Corning BioCoat cell culture inserts to assist you with achieving clear and accurate results.
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Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of…
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Corning® 500 mL RPMI 1640, 1X
CorningCorning glutagro™, phenol red Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy’s 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and…
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Corning BioCoat cell culture inserts are pre-coated with extracellular matrix proteins for applications requiring a protein-coated cell surface, such as cell adhesion, growth, invasion, migration and/or differentiation. Coatings include Corning Matrigel® matrix, Fibronectin, Collagen, and Laminin.
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Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells. This research indicated that additions made to BME aided cell propagation. MEM is modified with…
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Dulbecco's Phosphate Buffered Saline 1X w/o Phenol red, Calcium and Magnesium. Commonly used for tissue disaggregation and monolayer dispersal.
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Corning BioCoat cell culture inserts are pre-coated with extracellular matrix proteins for applications requiring a protein-coated cell surface, such as cell adhesion, growth, invasion, migration and/or differentiation. Coatings include Corning Matrigel® matrix, Fibronectin, Collagen, and Laminin.
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Sodium bicarbonate Solution 7.5%
HiMediaSodium bicarbonate Solution 7.5%. Used as a biological buffer in cell culture media to maintain physiological pH.
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Thomas Nos. B003K38 and B003K39 Iscove's Modification of DMEM (IMDM) is highly enriched synthetic medium optimized for rapidly proliferating cultures of high cell density. A modified form of Dulbecco's Modified Eagle Medium (DMEM), Iscove's contains additional amino acids,…
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MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the…
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Buffers
CorningSodium bicarbonate Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7.2 - 7.4 in a culture environment. Bicarbonate is a weak buffer with that makes maintaining a pH range of 7.2 - 7.6 somewhat difficult, but it is non-toxic, and is a…
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Cellvation Cryopreservation Media
MP BiomedicalsCellvation Cryopreservation Media is animal component free and has been shown to preserve the potential of stem cell for differentiation. This medium is recommended for the preservation of stem cells, hepatocytes, tissue samples, and a wide variety of cell types and established cell lines. …
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Dulbecco’s Phosphate Buffered Saline 10X is a stock solution used to prepare 1X D-PBS in cell culture grade water. D-PBS is used to wash cells during preparation and serial transfer Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding…
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Cefinase™ discs are intended for use in rapid testing of isolated colonies of Neisseria gonorrhoeae, Staphylococcus species, Haemophilus influenzae, enterococci and anaerobic bacteria for the production of β-lactamase.
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Poloxamer 188
CorningSpecialized for use in insect cell culture Poloxamer 188 is a surfactant used in cell culture to protect cells in suspension against possible damage during transfer, freezing and thawing, and stirring. It is a non-ionic detergent with an average molecular weight of 8400Da consisting of a central…
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Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…
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Liquid Blocker–Super PAP Pen
Electron Microscopy SciencesThe Newest Staining Guard Pen New and improved. The Super PAP Pen outperforms the original PAP Pen. The Super PAP Pen may be used at temperatures as high as 120°F. (The old style PAP Pen worked only up to 90°F). With this high temperature feature, one can now use the Super PAP Pen to…
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Fro-Marker
Electron Microscopy SciencesA wide tipped pen designed for use with frozen sections. The pen provides a sticky membrane which when applied to the slide and onto which the section is placed it eliminates the section from falling off, moving, or wrinkling on the slide during immunostaining procedures. The membrane is stable up…
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This Tryptic Soy Broth (TSB) product is offered for use in sterility testing and validation in the manufacture of a liquid product. The use of this medium in place of the product intended for production allows an accurate assessment of sterility throughout the manufacture process. The product is a…
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Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…
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EDTA Disodium Salt, 2% Solution
MP BiomedicalsEDTA solution for use as a gentle non-enzymatic cell dissociation reagent.
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Cell Freezing Medium B
Quality BiologicalCell Freezing Medium A protects cells from damage after freezing and thawing. Applications Cell Freezing Medium A is frequently used in cell biology research for the cryopreservation of cells. Cell Freezing Medium A (2X) works with Cell Freezing Medium B (2X). Recommendation Suspend…
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Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…
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Corning® glutagro™ DMEM
CorningDulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of…
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Other Corning® ECMs
CorningCorning extracellular matrices (ECMs) enable researchers to mimic in vivo environments for 2D and 3D cell culture applications.
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Type IV Collagen is a ubiquitous component in basement membranes and provides the major structural support for this matrix. When the Collagen IV meshwork is assembled, it provides a scaffold for the assembly of other basement membrane components through interactions with laminin, entactin/nidogen,…
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Corning insectagro Sf9 is formulated to support the propagation of Sf9 insect cells in culture, and can also be used for SF21 cells. The Sf9 cell line originated at the USDA Insect Pathology Laboratory from IPLBSF-21 cell line, derived from the pupal ovarian tissue of the fall army worm. Sf9 cells…
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20X PBS used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt…
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CMRL media are based on the composition of Medium 199. Nucleic acid derivatives, coenzymes omitted in H597 modification were retained in CMRL 1066. CMRL 1066 is a chemically defined medium formulated for long term cultivation of mouse L cells in the absence of protein supplement. Although…
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NanoShuttle-PL Refills
Greiner Bio-OneNanoShuttle™-PL consists of gold, iron oxide, and poly-L-lysine. NanoShuttle™-PL magnetizes cells by electrostatically attaching to cell membranes during an overnight static incubation. Magnetized cells will appear peppered with dark nanoparticles after incubation. NanoShuttle™-PL…
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2X EMEM without Phenol Red
Quality BiologicalEagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations Without Phenol Red With Glucose and L-glutamine Applications EMEM (Eagle’s Minimum Essential Medium) has…
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Corning® Ham's F-12 Medium
CorningHam's F-12 media was originally formulated for the serum-free culture of CHO cells, and is based on Ham's F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids. This medium is also suitable for carcinoma cells, rat skeletal myoblasts,…