Benzonase® Nuclease, =250 units/µL, =90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

Synonym: Endonuclease from Serratia marcescens

CAS Number: 9025-65-4

EC Number: 231-791-2

Enzyme Commission (EC) Number: 3.1.30.2

MDL Number: MFCD00131010

Application
Used for the removal of nucleic acid from protein samples.

Benzonase nuclease, or endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no photolytic activity. Benzonase nuclease can also be used to prepare proteins in microcalorymetric experiments.

Biochem/physiol Actions
Digests native or heat-denatured DNA and RNA.

Legal Information
Benzonase® Nuclease is supplied by Merck KGaA and its affiliates.

Benzonase is a registered trademark of Merck KGaA

Unit Definition
One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ?A₂₆₀ of 1.0 in 30 min at pH 8.0 at 37 °C (reaction volume 2.625 ml).

Physical form
Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl₂, and 20 mM NaCl.