Taq DNA Polymerase from Thermus aquaticus is a thermostable DNA polymerase that is used for the DNA polymerase chain reaction (PCR) in order to amplify DNA sequences.
Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 5X Direct Loading buffer contains a densifying agent and a red-purple dye that allow to follow the migration of the sample during the electrophoresis.
Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.
One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74°C.