Targeting RdRp, N, E and RNase P
The COVID-19 Real-Time PCR Detection Kit is designed for specific and qualitative detection of SARS-CoV-2 RNA in specimens such as oropharyngeal swabs, nasopharyngeal swabs or sputum suspected of SARS-CoV-2. The product is intended for use by qualified laboratory personnel well trained in nucleic acid amplification techniques and in vitro diagnostic procedures.
Principle
The primers and probes are selected from the regions of RNase P, ORF1ab ,N gene and E gene. Three primer/probe sets are designed for specific detection of SARS-CoV-2. An additional primer/probe set to detect human RNase P gene (as an internal control) is also included. RNA extracted from respiratory specimens is reverse transcribed and amplified in a Real-Time PCR Thermocycler (such as Applied Biosystems 7500 Real-Time PCR Instrument). The fluorescent signal is emitted when the 5’ nuclease active domain of Taq polymerase degrades the probe anneals to target sequence between the forward and the reverse primers. During each cycle, the fluorescence intensity is increased with the additional reporter dye molecules released from their respective probes.
The COVID-19 Real-Time PCR Detection Kit also contains a positive control (Plasmid DNA) and a negative control for testing process monitoring.