The novel Z-VAD(OMe)-FMK inhibitor synthesized using proprietary technology is cell permeable and irreversibly binds to activated Caspases to block apoptosis. Apoptosis is a physiological form of cell death, which plays an important role in embryogenesis, cellular homoestasis, tissue atrophy, and removal of damaged and mutated cells. Caspases, which act as molecular chainsaws, are the central components of apoptosis response. Caspases are cysteine proteases that cleave after aspartate residue in their substrates. At least 7 of 14 known mammalian caspases have important roles in apoptosis.
Z-VAD(OMe)-FMK is an irreversible and cell permeable broad-spectrum Caspase Inhibitor. It has been shown to inhibit Fas-mediated apoptosis in Jurkat T cells and apoptosis in THP cells induced by diverse stimuli (including cycloheximide, thapsigargin, etoposide and staurosporine).
Key Applications: General Caspase Inhibitor, Apoptosis Inhibitor
Application Areas: Cell Viability Analysis
Research Areas: Caspase Activation
Product Type: Proteins, Enzymes & Peptides
Protein or Enzyme Type: Activators, Inhibitors & Substrates
Chemical Class: proteins, Enzymes & Peptides
Presentation: White Solid
Format: Solid
NOTES: Note: This inhibitor is designed as a methyl ester to facilitate cell permeability. If the intended use is on purified or recombinant enzymes, esterase should be added to generate the free carboxyl groups.
Mass Spec: M+1=467.9; HPLC: > 95 %; Chromatography: EtOAC: 80, Hex: 20, Single Spot, Rf:0.3; Peptide content: 78%
Amino acid sequence: Z-Val-Ala-Asp(OMe)-FMK; Z-VAD(OMe)-FMK
Solubility: Make a stock solution of 20 mM in high purity DMSO (>99.9%). Dissolve 1mg of Z-Val-Ala-Asp(OMe)-FMK in:107 µl DMSO = 20mM; 214 µl DMSO = 10mM; 428 µl DMSO = 5mM
Storage & Handling: Keep cool and dry. Storage at room temperature does not effect this compound, however we recommend refrigerate at +4°C. The solid product is stable in the desiccator at room temperature for 1 year. The stock solution is stable at -20°C freezer for 6-8 months. Keep sealed after removing from the freezer until its temperature equilibrates with room temperature.