Product is goat affinity purified antibody to human IgA (α-chain) and buffer salts.
IgA antibody is a secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. Thus, secretory IgA contributes to the humoral defense mechanism against the pathogens on mucosal surfaces Anti-Human IgA (α-chain specific) antibody specifically recognizes human IgA. Immunoglobulin A (IgA) is the primary antibody involved in mucosal immunity. It is identified by the presence of α-type heavy chains. IgA, which is composed of two subclasses IgA1 and IgA2, can exist in a dimeric secretory form found in mucous secretions.
This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. It is also used for IgA quantification by ELISA in various studies. Antibody dilution of 1:4000 was used for passive hemagglutination inhibition assay. It may be used to locate and identify the α-chain of human IgA by immunocytochemical and immunohistochemical (IHC) techniques.
The total protein is measured by absorbance (A280nm). Antibody titer is standardized by equivalence-point precipitation. Each affinity purified antibody is tested for purity at 10 mg/mL using immunoelectrophoresis. The product is goat IgG; no trace of albumin is detected.
The affinity purified antibody is prepared from the specific goat IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.
Suggested working dilution: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 1: 2,000 for ELISA
Concentration: Total Protein: 1.1 mg/mL; Active Antibody: 1.0 mg/mL
Key Applications: Ouchterlony double diffusion, Immunostaining
Application Areas: Immunoassays
Product Type: Antibodies
Antibody Type: Secondary Antibodies
Antibody Category: Polyclonal
Conjugate: Unconjugated
Host: Goat
Immunogen: Purified human IgA
Species Reactivity: It shows reactivity to human IgA; cross-reactivity to other species may exist. Antibody activity to IgG, IgM, or non-immunoglobulin serum proteins is not present.
Format: Affinity purified liquid
Sterilization of Solutions: Filtered through a 0.22 µm filter
Storage & Handling: Store unopened product at 2-8°C. For long term storage, aliquot and store with 40% glycerol (v/v) at -20°C.