In addition to direct labelling, the secondary antibody can be labelled with biotin. Biotin itself cannot produce a detectable signal, but it binds strongly to streptavidin, which can be labelled with a variety of molecules, including enzymes, radioisotopes, fluorescent dyes, and gold. Antibodies…

The conjugation of horseradish peroxidase and streptavidin is achieved using the periodate coupling method described by Nakane and coworkers. This product is sold on the basis of titer in an ELISA and performance in a Western blot. As a result the final protein concentration of the conjugate…

Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents. Highly species-specific. Optimized for use with our range of Amersham ECL Western blotting detection reagents. Recommended dilutions to minimize background. Highly…

This goat anti-rabbit IgG HRP is used as the 'secondary antibody' for western blotting or ELISA where the primary antibody was generated in rabbits. This peroxidase-conjugated secondary antibody is suitable for western blotting when employing detection by commercially available…

The antibodies are supplied with dilution guidelines for relevant applications. Highly species-specific. Applications: Immunodetection, ELISA tests, in situ hybridization. Highly species-specific.Applications: Immunodetection, ELISA tests, in situ hybridization.The antibodies are supplied…

Streptavidin-Alkaline Phosphatase Conjugate Streptavidin conjugated to reporter enzyme molecules for use in blotting and in situ hybridization applications. High specificity reagents. Streptavidin conjugated to reporter enzyme molecules for use in blotting and in situ hybridization…

Western blotting is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. Proteins are separated via gel electrophoresis and transferred to a membrane. The proteins are visualized with antibodies specific to the target under investigation.…

Applications: (+) FC, IF, and WB

This goat anti-mouse IgG HRP is used as the 'secondary antibody' for western blotting or ELISA where the primary antibody was generated in mice. This peroxidase conjugated secondary antibody is suitable for western blotting when employing detection by commercially available…

We offer a range of secondary antibodies conjugated to either alkaline phosphatase (AP) or horseradish peroxidase (HRP). The antibodies are isolated from antisera by immuno-affinity chromatography using antigen coupled to sepharose beads or other methods. They are supplied lyophilized, from a…

This goat anti-mouse (IgG+IgM) FITC is used as the 'secondary antibody' for immunofluorescent staining on tissues or culture cells where the primary antibody was generated in mice. It is highly recommended that proper negative controls, such as omitting the primary antibody, be included in…

This goat anti-rabbit IgG FITC is used as the 'secondary antibody' for immunofluorescent staining on tissues or culture cells where the primary antibody was generated in rabbits. It is highly recommended that proper negative controls, such as omitting the primary antibody, be included in…