Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for the identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent. Working concentration: 50 µg/ml - 1 mg/ml for mammalian cell selection.
Synonyms: Hygromycin B, Streptomyces sp., Cell Culture-Tested - CAS 31282-04-9 - Calbiochem
Molecular Formula: C20H37N3O13
Molecular Weight: 527.52
Linear Structural Formula: C20H37N3O13
MDL Number: MFCD06795479
Purity: >=90% (HPLC and TLC)
Storage: 2-8C
Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes: Provided as a light brown lyophilized solid. Not provided under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 micron filter before use.