Heparin Separopore® 4B is an affinity matrix widely used in affinity purification of various heparin-binding proteins or ligands, such as antithrombin III, lipoprotein, DNA binding proteins (transcription factors, virus coat proteins etc.) and steroid receptors. It can also be used as a high capacity cation exchange medium. Specific proteins can be separated by using different concentrations of salt or a salt gradient. Heparin-Separopore® exhibits excellent binding capacity, a high flow rate, and no significant loss of the heparin ligand. Heparin Separopore® 4B contains heparin (from porcine intestinal mucosa) immobilized by CNBr activation coupling method.