The Oligo Clean & Concentrator provides a streamlined method for efficient recovery and clean-up of DNA fragments and oligonucletides16 nt from labeling (radioactive, biotin, DIG, etc.) and other enzymatic reactions. Unincorporated nucleotides, short oligos, dyes, enzymes, and salts are effectively removed by the clean-up procedure.
There is no need for organic denaturants or chloroform. Instead, the kit features Zymo-Spin column technology and employs a single-buffer system that allows for efficient DNA adsorption to the matrix of Zymo-Spin IC Column. DNA is washed and concentrated into a small volume of water (≥6 micro liter). Purified DNA, available in just 2 minutes, is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.