| Key Applications: | Nucleic acids extracted from MPure 12 Viral/Pathogen Nucleic Acids Extraction kit B can be used in a number of downstream application including: PCR, qPCR, Sequencing(NGS), Microarray, RFLP, Southern Blot Analysis, RT-PCR, DDRT PCR, TaqMan® Analysis, Northern Blot AnalysisNorthern blotting. |
| Application Areas: | Nucleic Acid Purification - viral and bacterial DNA/RNA from swab samples (cell-rich samples). |
| Research Areas: | Molecular Biology, Nucleic Acid Purification |
| Product Type: | Kits & Assays |
| Instrument or System Type: | Nucleic Acid Purification |
| Kit or Assay Type:: | Reagent Kit for Nucleic Acid Purification |
| Components: | Proteinase K = 40 µl, Lysis Buffer 2 = 720 µl,Binding Buffer 1 = 720 µl,Magnetic Bead Solution = 800 µl,Washing Buffer 1 = 1000 µl,Washing Buffer 2 = 1000 µl,Washing Buffer 3 = 1000 µl,Elution Buffer 1 = 1000 µl,Elution Buffer 2 = 1000 µl; BL2 Buffer = 400 µl |
| Instrument Compatibility: | Use with MPure-12 System |
| Material of Construction: | Plastic, Aluminum Foil and Buffers |
| Sample Type: | Viral, Bacterial and Swab samples |
| Analyte(s) Detected: | DNA and RNA |
| Sample Volume: | Bacteria Pellet: 100-200 µl /Up to 109 bacteria (about OD600 = 3); Bacterial Colony: 100-200 µl /1-3 colony; Swab samples: 100-200 µl liquid transport media |
| Capacity: | Bacterial Pellet: 100-200 µl /Up to 109 bacteria (about OD600 = 3); Bacterial Colony: 100-200 µl /1-3 colony; Swab Samples: 100-200 µl liquid transport media |
| Sample Throughput: | 1-12 samples and 48 Preps/Kit |
| Molecule Enriched: | RNA/DNA |
| Materials Required (Not Supplied): | Pipettes, Pipette Tips, Test Tubes, Gloves |
| Storage & Handling: | MPure-12 Viral/Pathogen Nucleic acids Extraction Kit B should be stored at room temperature (15-25deg;C). Do not freeze the reagent cartridges. The Kits are stable for 12 months under the condition. Store the purified Nucleic acids at 4 deg;C (short- term) or aliquot and store at -70deg;C (long-term) before perform the downstream analysis. |
| Certifications: | CE |
| Dispense Volume Range: | 50-300 µl |