Wilkins Chalgren Anaerobic Broth Base is used for cultivation and susceptibility testing of anaerobic bacteria. This medium formulated by Wilkins and Chalgren, is the preferred medium for susceptibility testing of anaerobes. This medium is also recommended for testing anaerobic bacteria. This media needs to be appropriately supplemented to support the growth of certain anaerobic bacteria. Hemin and Menadione (Vitamin K3) enhances the growth of Bacteroides species and Prevotella melaninogenica, respectively and many other species of gram-negative anaerobic rods. The medium can also be supplemented with defibrinated or lysed blood for the growth of fastidious anaerobic bacteria.