Sabouraud Chloramphenicol Agar is cited as Medium C and recommended for cultivation of yeasts and moulds. This medium was described originally by Sabouraud (1) for the cultivation of fungi, particularly useful for the fungi associated with skin infections. The medium is often used with antibiotics such as Chloramphenicol (2) for the isolation of pathogenic fungi from materials containing large numbers of fungi or bacteria.
Casein enzymic hydrolysate and peptic digest of animal tissue provide nitrogenous compounds. Dextrose provides an energy source. Chloramphenicol inhibits a wide range of Gram-positive and Gram-negative bacteria which makes the medium selective for fungi (3). The low pH favours fungal growth and inhibits contaminating bacteria from clinical specimens (4).
Storage and Shelf-life:
Store dehydrated medium and the prepared medium at 2 - 8°C. Use before expiry date on the label
References:
1. Sabouraud K., 1892, Ann. Dermatol. Syphilol, 3:1061.
2. Ajello L., 1957, J. Chron. Dis., 5:545.
3. Lorian (Ed.),1980, Antibiotics In Laboratory Medicine, Williams and Wilkins, Baltimore.
4. Murray, P. R 2005, In Manual of Clinical Microbiology, 7th ed., ASM, Washington, D.C.