Dissolve 8 g of the medium in a liter of deionized or distilled water. Dispense as desired and sterilize at 121°C (15 lbs. of pressure) for 15 minutes.
Nutrient Broth is used in many laboratory procedures as is, or with added indicators, carbohydrates, organic liquids, salts, etc. This medium is used in accordance with official recommended procedures for the bacteriological analyses of water, milk and dairy products, in foods of important sanitation, tests for sensitivity and resistance and as a base to prepare media supplemented with other nutrients. It can be used for enumeration, isolation and enrichment as well as providing a high grade base for the preparation of special media. With the addition of blood, ascites fluid or serum, they can also be used to cultivate streptococci, pneumococci and erysipelas species etc.
Nutrient Broth is a general purpose medium used for cultivating a broad variety of fastidious and non-fastidious microorganisms with non-exacting nutritional requirements. Peptone and yeast extract provide nitrogenous compounds, vitamin B complex, amino acids and other essential growth nutrients. Sodium chloride is used to maintain the osmotic balance and glucose is the fermentable carbohydrate source of the media.
Key Applications: Growth of fastidious and non-fastidious microorganisms
Product Type: Culture Media
Cells Tested: The following typical results were obtained in the performance of the medium from type cultures after incubation at a temperature of 37°C and observed after 24 hours: Enterobacter aerogenes ATCC 13048: good to excellent; Escheria coli ATCC 25922: good to excellent; Salmonella typhi ATCC 6539: good to excellent; Staphylococcus epidermis ATCC 14990: good to excellent; Streptococcus pyogenes ATCC 12344: Moderate
Presentation: Tan Powder
Format: Powder
Formulation: Nutrient Broth is a liquid medium, produced according to the formula from APHA and AOAC. Approximate formula: Gelatin Peptone: 5.0 g/L; Beef Extract: 3.0 g/L; Final pH: 6.9 +/- 0.1
pH: 6.7 - 7.1 (8 g/L)
Solubility: Soluble in water (8 g/L).
Sterilization of Solutions: Sterilize at 121°C (15 lbs. of pressure) for 15 minutes.
Storage & Handling: Store at Room Temperature (15-30°C)