Single dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and dropout formulations.
Preparation Method
Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the DOB (or DOBA) with CSM-Trp+Ade formula. DOB (Drop Out Base) is 1.7g Yeast Nitrogen Base, 5g Ammonium Sulfate, 20g Dextrose per liter. DOBA is DOB with 17% agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid medium (or plate medium) by adding 2.7g DOB (or 4.37g DOBA) powder and 0.076g of CSM-Trp+Ade to a 100 mL of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121°C for 25 minutes. After cooling to 50°C, plates of DOBA with CSM-Trp +Ade were poured.
Quality Control Assay
DOB (or DOBA) with CSM-Trp+Ade was visually inspected after autoclaving for complete dissolution of components. The pH of the medium was tested to insure that the proper specification was attained. Acceptable pH range is 4.0-4.8 for DOB with CSM-Trp+Ade, report result for DOBA with CSM-Trp+Ade. DOB (or DOBA) with CSM-Trp+Ade was tested by S.cerevisiaecell growth at 30°C for approximately 48 hours.