NUCLEOSIL® is a family of totally porous spherical silicas with a very pure and uniform SiO2 structure
- Wide acceptance as routine packings for very different fields of chromatography
- One of the first spherical silicas used in HPLC
- Developed in the early seventies, it became a world-renowned HPLC packing
- Still found in many analytical and preparative application
- An absolutely reliable choice in HPLC
- The largest variety of modified HPLC silicas available on the market
NUCLEOSIL® PPN
Silica-based HPLC phases for RP chromatography of biological macromolecules
Features
- Silica-based reversed phase materials with polymerically bonded alkyl chains, exclusively hydrophobic interactions
NUCLEOSIL® 100-5 C18 PPN: octadecyl phase · USP L1
- Dynamic protein binding capacity per g packing: 8 mg BSA, 64 mg cytochrome C
- Suited for the separation of peptides and proteins up to about 40 kD, also suited for basic peptides
- Particle size 5 µm
- Pore size 100 Å
- pH working range 1–9
- Max. working pressure 250 bar
Eluent in column methanol
NUCLEOSIL® 500-5 C18 PPN: octadecyl phase · USP L1
- Dynamic protein binding capacity per g packing: 22 mg BSA, 40 mg cytochrome C
- Especially suited for large peptides and medium-size hydrophilic proteins
- Particle size 5 µm
- Pore size 500 Å
- pH working range 1–9
- Max. working pressure 250 bar
Eluent in column methanol