Application: Broadly used in molecular biology and cell culture as a selection agent for prokaryotic cells that have been transformed using the selectable marker gene (neo). Recommended for use in cell culture applications at 5 ml/L.
Other Notes: Formulated to contain 10 mg/ml neomycin in 0.9% sodium chloride.
Caution: Stable at 37 °C for 5 days.
Storage: 2-8°C
Biochem/physiol Actions: Mode of action: binds to the 30S and in some cases the 50S subunit causing miscoding; inhibits initiation and elongation during protein synthesis.
Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.
Empirical Formula (Hill Notation): C13H12O