Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor. The ligation will occur only if the oligonucleotides are perfectly paired to the complementary target DNA and have no gaps between them; therefore, a single-base substitution can be detected. Taq DNA Ligase is active at elevated temperatures (45°C-70°C)
Product Includes
Taq DNA Ligase
10X Taq DNA Ligase Buffer with NAD+
• Useful for Allele-specific gene detection via LCR and Mutagenesis by incorporation of a phosphorylated oligonucleotide.
• Extremely high purity.
• Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product.
• The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining.