CAS Number: 9050-94-6
MDL Number: MFCD00132235
Features and Benefits
- Excellent recovery
- Minimal sample dilution
- Quick and easy to use
General Description
Sephadex® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large moleculaes. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.
Application
Sephadex® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.
Legal Information
Sephadex is a registered trademark of Cytiva .
Grade: DNA grade; for molecular biology
Product Line: BioReagent
Form: beads
Swelling: 1 g swells to 9-11 mL
Bead Diameter: 40-120 μm
Pore Size: exclusion limit (25 bp dsDNA; (rA)27)
pH: 2 - 10
Foreign Activity: DNase, RNase, none detected
Storage Temp.: room temp