The MISSION pLKO.1-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 mug of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/mul.
Synonyms: MISSION® Control Vectors; negative control; negative shRNA control; non-target control; non-target shRNA; non-target shRNA control; shRNA control
MDL Number: MFCD07785395
Storage: -20C
Application: MISSION® pLKO.1-puro non-target shRNA control plasmid DNA has been used as a control during transduction:
•in tumor cells for multicolour imaging
•in human adult low calcium temperature keratinocytes
•in mouse embryonic fibroblasts, to study the biological functions of IP6K1 (inositol hexakisphosphate kinase)
•to study the function of Zac1 (zinc finger protein regulating apoptosis and cell cycle arrest) expression in astroglial differentiationTo see more application data, protocols, vector maps visit sigma.com/shrna.
Legal Information: Use of this product is subject to one or more license agreements. For details, please see issionlicense">http://sigmaaldrich.com/missionlicense.