Epidermal growth factor or EGF is a growth factor isolated from mouse submaxillary glands that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa and SV40-3T3 cells.
EGF stimulates the proliferation and differentiation of a wide variety of cell types deriving both from ectoderm and mesoderm. In tissue culture, EGF can act to reduce or eliminate the requirement for serum and is often used in conjunction with other media additives and hormones, such as insulin, transferrin, and prostaglandin E1. Cellular metabolic effects of EGF include stimulation of ion fluxes, glucose transport, glycolysis, and synthesis of DNA, RNA and proteins.
The sample should be dissolved in sterile water or a sterile albumin supplemented buffer. Solutions of pure EGF should be aliquoted and lyophilized, while solutions containing albumin may be stored at -20°C.
Grade: Tissue Culture Grade
Purity: ≥95%
Suitability: Suitable for tissue culture
Key Applications: Stimulates proliferation of cells
Application Areas: Molecular Biology
Product Type: Proteins, Enzymes & Peptides
Presentation: White Solid
Format: Solid
Formulation: Lyophilized from a solution of 0.01M sodium acetate
NOTES: Purified by a modification of the procedure of Cohen and others. Electrophoreticaly homogenous (Isoelectric focusing, Malik and Barre stain).
Storage & Handling: Store at +4°C. Store dessicated.