MOPS is used as a biological buffer.
MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. It is a structural analog to MES; however, MOPS is a more suitable biological buffer than MES because the pH range is closer to pH 7.4. A buffer using MOPS sodium salt can be made by mixing equimolar MOPS free acid and MOPS sodium salt solutions to attain a buffer of the desired pH. Titration of a solution of MOPS sodium salt with HCl results in a solution that will contain NaCl, so the ionic strength will be higher than appropriate for some applications.
Concentrations higher than 20 mM should not be used with mammalian cell cultures.
Key Applications: Buffer
Product Type: Biochemicals
Biochemical Category: Buffers
UV/Visible Absorbance: OD250 (1% aq soln) <0.05
Presentation: White Powder
pH: 9.0 - 10.5(1% aq solution), useful pH range 6.5 - 7.9
pKa: pKa-7.2 at 25°C
Water Content: ≤0.5%
Solubility: Soluble in water (to approximately 33% w/w - clear, colorless solution).
Storage & Handling: Room Temperature