For quantitative determination of Mg2+ and evaluation of drug effects on magnesium metabolism.
- Linear detection range 0.1 - 3 mg/dL (41 - 1200 uM) Mg2+ in 96-well plate assay.
- Can readily automated to process thousands of samples per day.
- Assays can be executed in microplate or cuvet.
- Method: OD500 nm.
- Samples: Biological, food and beverage.
Magnesium (Mg) is one of the most abundant and essential minerals in mammals.Magnesium is involved in more than 300 biochemical reactions in the body and plays important roles in muscle and nerve functions, heart rhythm, immune system and bone formation. Magnesium deficiency may lead to nausea, fatigue, muscle contractions, hypocalcemia and hypokalemia. Simple, direct and automation-ready procedures for measuring magnesium concentration in biological samples are becoming popular in Research and Drug Discovery. BioAssay Systems magnesium assay kit is designed to measure magnesium directly in biological samples without any pretreatment. A calmagite dye in the kit forms a colored complex specifically with magnesium. The intensity of the color, measured at 500 nm, is directly proportional to the magnesium concentration in the sample. The optimized formulation minimizes interference by potential substances.