IL-1 alpha, Interleukin-1 alpha, murine (mouse): Murine Interleukin-1 alpha
Interleukin-1 alpha (IL-1α) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, hematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. Among various species, the amino acid sequence of mature IL-1α s conserved 60 % to 70 % and human IL1 has been found to be biologically active on murine cell lines .
Source: Escherichia coli.
Molecular Weight: Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.
Quantity: 2µg/10µg/1000µg
AA Sequence: SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS
Purity: > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of > 5.0 × 108 IU/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4.
Endotoxin: Less than 1 EU/µg of rMuIL-1α as determined by LAL method.