The DNA-Pure Yeast Genomic Kit provides a rapid, 30 minute protocol for the isolation of high quality, intact yeast genomic DNA. This procedure eliminates the need for enzyme digestions and organic extractions which can result in sheared, variable quality DNA. The DNA obtained using the DNA-Pure Yeast Genomic Kit provides for excellent restriction digestion and PCR results.
Typical yields from this kit are 10 - 20 ug DNA from a 1.5-3 ml culture. The kit has been tested successfully with Saccharomyces cervisiae, Schizosaccharomyces pombe, and Pichia pastoris. The Kit utilizes an easy to follow protocol: 1) pellet cells; 2) resuspend cells and add lysis solution; 3) add protein solubilization solution after a 10 minute incubation at 70 C; 4) centrifuge and then isopropanol precipitate DNA from the supernatant; 5) wash pellet and resuspend in TE or water.
Advantages:Easy 30 minute protocol High yield of quality, intact yeast genomic DNA. No lytic enzymes or proteinase K needed. No organic extractions. DNA can be used directly for restriction enzyme digestion, PCR, and hybridization.
Components:
Resuspension Buffer I, Lysis Buffer, Protein Solubilization Buffer, Resuspension Buffer II.