Trichloroacetic acid is an acetic acid analogue commonly used to precipitate proteins, DNA and RNA. In the presence of SDS Trichloroacetic acid will precipitate proteins which can then be quantified by the Lowry method. The compound has also found uses as a decalcifier and fixative in microscopy, in protein sequencing, detecting albumin and organic synthesis.
Trichloroacetic acid is used in protein precipitation; has been used to determine protein concentration by quantitative precipitation. It is also used as a decalcifier and fixative in microscopy. A protocol for the precipitation of nucleic acids can be found in Molecular Cloning. Rats exposed to chronic sublethal amounts of trichloroacetic acid displayed an increase in serum bilirubin with a decrease in hematological proteins and cholesterols along with significant decreases in red blood corpuscles, mean cell volume, mean corpuscular hemoglobin, hemoglobin and hematocrit. In mice the production of liver tumors was associated to Trichloroacetic acid, which is also a known mouse hepatocarinogen. On the guinea pig trichloroacetic acid was observed to be a mild alergen on the skin.
Assay: ≥99%
Key Applications: Decalcifier, Fixative, Determine protein concentration, Precipitation of Proteins
Product Type: Organics
Chemical Class: Organic Acids & Bases
Density: 1.61 g/cm3 (Lit.)
Boiling Point: 197°C at 1013 hPa(Lit.)
Melting Point: ca. 56°C(Lit.)
Vapor Density: 5.6 (air = 1)(Lit.)
Vapor Pressure: 1.2 hPa at 50°C(Lit.)
Refractive Index: n20/D 1.62(Lit.)
Presentation: Powder
Format: Powder
pH: 1.2 (0.1 M aqueous solution)(Lit.)
NOTES:
Poison/Deleterious Substance Code: 2-58
Chloride= ≤0.002%
Solubility: Soluble in water(10% aq soln)- Clear, Colorless Solution; very soluble in ethanol or ether.
Storage & Handling: Room Temperature, desiccate
Certifications: Protein precipitation reagent