Tissue Freezing Medium

TFM™ Tissue Freezing Medium Embedding matrix for frozen sections. Unique formula reduces curling, allowing easier pickups of flat serial sections. Reduced water content minimizes freeze fractures. Color variety makes differentiating multiple specimens easy. Available in clear, transparent…

…for later fixation and staining. Drying or fixing the slides in the cryostat for specific procedures will not affect the PolyFreeze medium. Storage: room temp Application: Cryo-embedding matrix for frozen specimens.PolyFreeze tissue freezing medium has been used in immunohistochemistry.

… Application: A support matrix or form of embedding medium for frozen sectioning. This medium freezes quickly supporting the tissue for sectioning at 3 mu and up with no cracking of the matrix at temperatures from -8 C to -25 C. Snap freeze using PolyFreeze with isopentane and liquid nitrogen or…

Our SubZero-Matrix Tissue Freezing Medium freezes quickly without crystallization, specific formula reduces curling, allowing easier pickups of flat serial sections. Convenient flip cap for improved ease of use.

…Freezing Medium A (2X) works with Cell Freezing Medium B (2X). Recommendation Suspend pelleted cells in cold Cell Freezing Medium A. Transfer to a freezing vial and add equal parts of cold Cell Freezing Medium B. Mix well and store by appropriate methods. Accessory item: Cell Freezing Medium…

…Freezing Medium A (2X) works with Cell Freezing Medium B (2X). Recommendation Suspend pelleted cells in cold Cell Freezing Medium A. Transfer to a freezing vial and add equal parts of cold Cell Freezing Medium B. Mix well and store by appropriate methods. Accessory item: Cell Freezing Medium…

CryoXL™ Cell Freezing Medium 1X w/ FBS and DMSO w/o Antibiotics. Used for hardy cell lines that are less susceptible to freezing damage.

…Cell Expansion Medium (Cat. No. SCM015) and counted to determine viability and number. 4. Centrifuge cells at 300 x g for 3 min. Remove the medium above the pellet. 5. Resuspend the cells in Mesenchymall Stem Cell Freezing Medium at a concentration of ~4 x 106 cells/mL. Freeze 1 mL of cells/vial.…

…specificity for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases. It is designed to be used as a supplement to tissue culture medium for the prevention of secreted proteins degradation. The product has been found to be non-toxic after a 48 hours exposure to the following…

QBI's Serum Free Cryo Medium is a sterile, 2X concentrated freezing medium containing DMSO  developed for the cryopreservation of cells grown under serum free conditions. Serum Free Cryo Medium is an animal-free, protein-free, chemically defined medium containing DMSO and other…

Applications The FBS in the Cryopreservation Medium improves the post-freezing recovery and survival after cryopreservation, while DMSO preserves the cells during freeze. Cryopreservation Medium with Fetal Bovine Serum (FBS) and Calf Serum is a complete cryopreservation medium with 10% DMSO.

…capability of the stem cell populations.Organoids can be cryopreserved using standard cell freezing techniques however freeze/thaw viability remains a challenge. The 3dGRO Organoid Freeze Medium is a proprietary optimized cryopreservation media for multiple organoids cell types (etc. mouse…

An aqueous-based frozen section embedding medium designed to support tissue blocks in cryostat sectioning. Formulated to promote rapid freezing, enhanced sectioning and consistent results at a working temperature of -20C. 4 oz bottle.

…free of any mammalian or avian tissue-derived raw materials. Application: Liberase ™ (Thermolysin Medium) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial…

…mounting medium. The Kit will take the specimen from dye marking, cryo-embedding, melanin bleaching, staining and mounting medium to a finished slide which can be viewed under the microscope within minutes. Kit Contains: Tissue Marking Dye(5x2oz bottles), Tissue Freezing Medium (6x4oz bottles),…

…potential of stem cell for differentiation. This medium is recommended for the preservation of stem cells, hepatocytes, tissue samples, and a wide variety of cell types and established cell lines. Cellvation is a unique cryopreservation medium designed for optimal cell recovery and convenience.…

…to stimulate proliferation of the mouse T-cell line CTLL Filtered (0.2 µm membrane) and tested for bacteria, fungi and mycoplasma Source: Recombinant, murine T-cell line Molecular Weight: 17 kD Tip: Recommended concentration - 10 BRMP units/ml of medium depending on cell type.

…organic and inorganic compounds. In addition, DMSO is used in cell culture as a cryoprotectant added to cell culture medium, to preventformation of ice crystals during freezing of cells. DMSO can be used in molecular biology in PCR reactions to inhibit the self-complementation of DNA or PCR primers…

…Detection kit:Primary antibodies, PLA probes, wash buffers, mounting medium Other Notes: Storage/StabilityStore Box A and all of its components at -20C. The enzymes should be kept cold (-20C) at all times; Use a freezing block when removing them from the freezer.Store Box B and all of its…

…® is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid, effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting…

…for more information.Not included in Detection kit:Primary antibodies, PLA probes, wash buffers, mounting medium Preparation Note: Storage and Stability: Store the components at -20 C. The enzymes should be kept cold (-20 C) at all times, use a freezing block when removing them from the freezer.

…for more information.Not included in Detection kit:Primary antibodies, PLA probes, wash buffers, mounting medium Preparation Note: Storage and Stability: Store the components at -20 C. The enzymes should be kept cold (-20 C) at all times, use a freezing block when removing them from the freezer.

…to maintain the osmotic equilibrium of the medium. Dextrose serves as an energy source. The medium can also be enriched with 5 % v/v sterile defibrinated horse blood. For selective isolation of Brucella species antibiotic mixtures in the form of freeze dried supplements (FD) are incorporated into…

… is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid, effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting…

Storage: -20C Application: Primary Human Osteoblasts (HOB) are isolated from femoral trabecular bone tissue from the knee or hip joint region (lot specific source information is available on request). They stain positive for alkaline phosphatase and can be mineralized using PromoCell…

…electron coupling reagent immediately before use. Working instruction Cells are grown in microplates (tissue culture grade, 96 wells, flat bottom) in a final volume of 100 μl culture medium per well, according to the media needs of the cells in a humidified atmosphere ( e.g., 37 °C,…