T75 Flask

Advanced TC™ polymer modification for fastidious or sensitive adherent cells Free of detectable DNase, RNase, human DNA Non-Pyrogenic, non-cytotoxic Growth area: 75 cm 2 Total Volume: 250 mL Working volume: 15 ml - 38 mL With blue standard screw cap Sterile …

…of serological pipettes and cell scrapers. Flasks are divided into 6 types, depending on the culture scale and the cap type. Cell growth area for T25, T75, T175 and T225 are 25 cm², 75 cm², 175 cm² and 225 cm², respectively. Each flask can be provided with plug or filter caps …

Corning’s enhanced T-75 Flask features a U-shaped design, which improves usability while maintaining the same environment for cell growth as previous designs. The U-shaped T-75 flask includes design advances, such as rounded shoulders, which reduce the number of corners and improve cell…

…an airflow rate of 4.5 (LPM/3.7cm2/13.5psi), the gas exchange properties of the filtered flasks are comparable to that of the standard cap flasks. Synonyms: T75 flask; cell culture flask; tissue culture flask Legal Information: TPP is a registered trademark of TPP Techno Plastic Products AG

…of recombinant lentiviruses from lentiviral-transfected cell culture supernatant. Viral particles can be purified from cell culture of 4 to 5 T75 flasks per column. The viruses are first applied to a purification column and then further purified and concentrated through a concentration unit.…

…Flasks. They are available in three sizes: T–25, T–75 and T–175 with TC treated or non–treated surfaces. Filter caps with innovative air filter technology or plug–seal caps with arrested position to prevent undesired closing are the two closing options. Flasks are…

…Adenovirus from adenovirus transfected cell culture supernatant. Up to 1 x10 12 viral particles can be purified from cell culture media of 1 to 2 T75 flasks. Traditionally the recombinant adenovirus is purified by ultra centrifugation using CsCl to separate the virus particles from cellular…

…from retrovirus-transfected cell culture supernatant. Up to 1 x10 13 viral particles can be purified from cell culture media of 6 to 8 T75 flasks. Traditionally the recombinant retrovirus is purified by ultracentrifugation using CsCl to separate the virus particles from cellular proteins…

…from retrovirus-transfected cell culture supernatant. Up to 3 x10 12 viral particles can be purified from cell culture media of 1 to 2 T75 flasks. Traditionally the recombinant retrovirus is purified by ultracentrifugation using CsCl to separate the virus particles from cellular proteins…

…Adenovirus from adenovirus transfected cell culture supernatant. Up to 4 x10 12 viral particles can be purified from cell culture media of 6 to 8 T75 flasks. Traditionally the recombinant lentivirus is purified by ultra centrifugation using CsCl to separate the virus particles from cellular…

…from lentiviral-transfected cell culture supernatant. Up to 3x10 10 viral particles can be purified from cell culture media of 1 to 2 T75 flasks. The viruses are first pelleted from viral supernatant and then further purified and concentrated through a purification column and a…

…from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels: . 6 x 60mm dishes . 2 x T75 flasks . 2 x 6 well plates Refer to Murine Embryonic Stem Cell Culture Manual for complete culture protocols and media formulations for PMEF cells. …

…Optimized height, outstanding quality and specifications make these conical tubes ideally suited for cell biology e.g. for pooling media from T75 flasks, bacteria culture in midi prep size or overlapping applications like buffer preparation, storage and transport. Discover the new Eppendorf…

…Fully coat the cell culture surface with diluted Poly-L-Lysine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks.4. Allow cell culture vessel to sit at room temperature overnight.5. Aspirate the Poly-L-Lysine solution the following day and rinse vessel…