Roche Dnase I

…Recombinant DNase I is a DNA-specific endonuclease.The enzyme catalyzes the degradation of both double- and single-stranded DNA randomly by hydrolyzing phosphodiester linkages to DNA, resulting in a mixture of oligo- and mononucleotides. All material used during the production process of DNase I…

…cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping. General description: Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting…

…deoxyribonuclease I (DNase I) has been used for- • The isolation of cells from lung, skin and tumor samples Bacterial DNA extraction from live bacterial cells that are resistant to DNase I † General description: Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA…

…Mg 2+ DNase I causes single-stranded nicks in dsDNA, while in the presence of Mn 2+ the enzyme produces double-stranded breaks. Storage conditions (working solution): Long-term Storage of the Dissolved Enzyme The solvent generally recommended for DNase I is water. When DNase I is reconstituted…

…spermidine) Storage conditions (working solution): Solution is stable to at least 12 months if stored at 2 to 8 °C, contact with DNase or bacteria is to avoid. Sequence: In an aqueous solution the DNA itself is a salt (the acid groups of the phosphate back bone have a negative…

…glycerol (v/v), DNA Polymerase I and DNase I. Specificity: Heat inactivation: Stop the reaction by adding 1 μl 0.5 M EDTA (pH 8.0) and heating to 65 °C for 10 minutes. Principle: The nick translation method is based on the ability of DNase I to introduce randomly distributed…

…μl 0.4 mM; dTTP, 50 μl 0.4 mM; Nick Translation Buffer, 100 μl 10x concentrated; Enzyme Mixture, 100 μl, DNA Polymerase I and DNase I in 50% glycerol (v/v) RIDADR: NONH for all modes of transport Application: The kit is used for labeling of DNA with radioactive or modified…

…is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems,…

…ATP, in Tris buffer 10 mM; CTP, in Tris buffer 10 mM; GTP, in Tris buffer 10 mM; UTP, in Tris buffer 10 mM; Transcription Buffer 10x concentrated; DNase I, RNase free, in buffer with 50% glycerol; RNase Inhibitor, in buffer with 50% glycerol; SP6 RNA Polymerase, in buffer with 50% glycerol; T7 RNA…

…Polymerase 20 U/μl; T7 RNA Polymerase 20 U/μl; T3 RNA Polymerase 20 U/μl; Anti-Digoxigenin-AP antibody, Fab fragments 750 U/ml; DNase I, RNase free 10 U/μl; CDP- Star ready-to-use; Actin RNA Probe, DIG-labeled Antisense Probe, length 588 bases 10 ng/μl; DIG Easy Hyb…

…0.2 and 15 kb in: Analysis of PCR products • Examination of restriction endonucleases • Digests of plasmid, cosmid, and λ phage DNA • Electrophoresis of RNA in, e.g. , denaturing gels containing formaldehyde Nucleic acid fragments separated with Agarose LE…

…no amplification product is obtained. Components: • Lysis/Binding Buffer • DNase I, lyophilizate • DNase Incubation Buffer • Wash Buffer I • Wash Buffer II • Elution Buffer • High Pure Spin Filter Tubes (containing…

…digested with DNase I directly on the filter. Brief wash-and-spin steps readily remove the digested DNA fragments and other contaminating substances. The remaining purified RNA is then eluted in a small volume of low-salt buffer. Legal Information: LightCycler is a registered trademark of Roche

…from the column, residual DNA is digested by incubating the eluate with DNase I. A second incubation step with Proteinase K improves the purity of RNA. Finally, a low-salt elution releases the RNA from the glass fiber fleece. Legal Information: LightCycler is a registered trademark of Roche

…on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases. The enzyme is also available as a solution. • I nhibitors : The enzyme is inactivated by Pefabloc ® SC. However, it is not inactivated by metal ions, chelating…

…Roche Applied Science.For nonradioactive labeling of in situ probes with other haptens, Roche Applied Science offers the DIG-Nick Translation Mix or the Nick Translation Mix. Physical form: 1 vial with 5x concentrated stabilized reaction buffer in 50% glycerol (v/v) and DNA Polymerase I, DNase I,…

…from Roche Applied Science. For nonradioactive labeling of in situ probes with other haptens and fluorophores, Roche Applied Science offers the Biotin-Nick Translation Mix and the Nick Translation Mix (without nucleotides). Principle: The nick translation method is based on the ability of DNase I…