Random Primers

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  • ROCHE Hexanucleotide Mix

    MilliporeSigma

    primer mixture, which leads to binding of primer to the template DNA in a statistical manner. Thus, an equal degree of labeling along the entire length of the template DNA is guaranteed. The complementary strand is synthesized from the 3′-OH termini of the random hexanucleotide primer using…

  • Storage: −20°C

  • …RNase inhibitor maintains sample integrity Easy reaction set up Quick, accurate and reproducible Two formulations: 5X super mix with an optimum blend of anchored oligo and hexamer primers Flex kit with separate oligo and random primer preparations and an advanced buffer

  • …3′-OH termini of the random oligonucleotides as primers. Other Notes: For life science research only. Not for use in diagnostic procedures. Quality: Function tested in combination with the DIG RNA Labeling Kit. Components: 5x concentrated random primer mix: 1U/μL Klenow…

  • …containing various lengths of cDNA. Random priming is incapable of distinguishing between mRNA and other RNA species present in the reaction. Primer Sequence: 5´ – d (NNNNNN) –3´ N = G, A, T or C A mixture of random hexamer primers and oligo(dT) may improve the…

  • …modified deoxyribonucleoside triphosphate using random oligonucleotides as primers. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow Polymerase using the 3′-OH termini of the random oligonucleotides as primers. Other Notes: For life science research…

  • …The primer hybridizes to the poly-adenylated tail found on the 3´ end of most eukaryotic mRNAs. Oligo (dT)18 ensures that the 3´ end of mRNAs are represented. Primer sequence: 5´-d (TTT TTT TTT TTT TTT TTT)-3´ A mixture of random hexamer primers and oligo(dT)…

  • …for first-strand cDNA synthesis reactions. For priming, three different primer systems can be used. Two cDNA synthesis primers are provided with the kit: random hexamer primers and an anchored-oligo(dT) 18 primer. The latter is designed to bind at the beginning of the poly(A) tail to generate…

  • …thermostability. The kit also provides two optimized primers and nucleasefree water. An anchored Oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail and the random hexamer primer mix provides random and consistent priming sites covering the entire RNA templates…

  • cDNA Synthesis & Go Kit

    MP Biomedicals

    …Buffer provides highly optimized components for efficient reverse transcription, and includes a unique blend of anchored oligo dT and random hexamer primers to ensure unbiased 3’ and 5’ coverage for enhanced data accuracy. An extremely efficient reverse transcriptase delivers highly…

  • ROCHE Primer "random"

    MilliporeSigma

    Storage: −20°C UNSPSC Code: 41106300 RIDADR: NONH for all modes of transport Application: Primer for cDNA synthesis General description: p(dN)6 Other Notes: For life science research only. Not for use in diagnostic procedures.

  • … Product Description Tetro™ cDNA Synthesis Kit contains our highly sensitive MMLV reverse transcriptase, oligo (dT)18 and random hexamer primers and all the necessary components to generate high quality cDNA from RNA templates (fig. 1). The first-strand cDNA generated is ideal for PCR…

  • …affinity, coupled with a novel TransAmp buffer system for improved yield of full-length cDNA Unbiased – optimized mix of random hexamers and anchored oligo dT primers for complete 5’ to 3’ RNA sequence representation Sensitive – lower Ct values from a broad range of…

  • …modified deoxyribonucleoside triphosphate using random oligonucleotides as primers. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Other Notes: For life science research…

  • …increased thermostability. The kit also provides two optimized primers and nuclease-free water. An anchored Oligo-dT primer forces the primer to anneal to the beginning of the polyA tail and the random hexamer primer mix provides random and consistent priming sites covering the entire RNA templates…

  • igScript™ RT-qPCR Kit

    Intact Genomics, Inc

    …require minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions.. Product Includes: 5x igScript™ master mix Oligo d(T)23 VN primer (50 µM) Random hexamer primer mix (60 µM) ig SYBR® Green qPCR 2x master mix Nuclease free water

  • …first-strand cDNA synthesis, with total RNA, mRNA and in vitro transcribed RNA and shows excellent performance with gene-specific primers, Oligo (dT) as well as random hexamers, making it perfect for cDNA library construction and the production of templates for RT-PCR analysis of gene expression.

  • igScript™ RT-PCR kit

    Intact Genomics, Inc

    …The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-PCR reactions.c Product Includes: 5x igScript™ master mix Oligo d(T)23 VN primer (50 µM) Random hexamer primer mix (60 µM) Hot start Taq 2x master mix Nuclease free water

  • …of transport Application: The First Strand cDNA Synthesis Kit can be used with either sequence-specific primers, poly(dT)15 primers, or random primers, or gene-specific primers (not part of the kit). It can be used for the: • Detection of the presence or absence of RNA…

  • …thermostability. The kit also provides two optimized primers and nucleasefree water. An anchored Oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail and the random hexamer primer mix provides random and consistent priming sites covering the entire RNA templates…

  • …RNA input High specificity Shorter PCR extension times with PfuUltra II High accuracy Kit includes dNTP, dTT, oligodT and random primers Capable of amplifying cDNA of 0.1–9.6 kb in length 10–1000 ng of total RNA or 0.1–10 ng of poly(A)+ RNA input …

  • ROCHE Biotin-High Prime

    MilliporeSigma

    … −20°C UNSPSC Code: 41106000 RIDADR: NONH for all modes of transport Features and Benefits: Contents 5x concentrated random primer mix: 1U/μl Klenow polymerase, labeling grade, 1mM dATP, 1mM dCTP, 1mM dGTP, 0.65mM dTTP, 0.35mM Biotin-16-dUTP, in 50% (v/v) glycerol. …

  • …double stranded DNA to form blunt ends. Mutagenesis of DNA with second strand synthesis using oligonucleotides. Labeling DNA by the random primer method Components: DNA Polymerase I is supplied as a solution in 50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 5 mM dithiothretol, and 50% glycerol…

  • …second-strand synthesis using the Gubler-Hoffman method. Primers are not included in the preassembled First-Strand Reaction Mixes, thus allowing use of a primer of choice. Two primers are included with the kit. pd(N)? Primer (random hexamer) is used to prepare libraries for screening with…

  • …labeling of DNA with Digoxigenin-dUTP using random oligonucleotides as primers. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Other Notes: For life science research only.…

  • … RIDADR: NONH for all modes of transport Application: Use Klenow Enzyme for: • Random-primed DNA labeling using random oligonucleotides as primers for the incorporation of nonradioactively labeled and 32 P-labeled nucleotides. • Fill-in reaction for blunt-end formation…

  • ROCHE High Prime

    MilliporeSigma

    …may require a prolonged incubation period of 30 to 60 minutes. Application: For the labeling of DNA with radioactive dCTP using random oligonucleotides as primers. High Prime-labeled probes are used in a variety of hybridization techniques: • Southern blots • Northern blots…

  • …labeling method which is based on the hybridization of random oligonucleotides to the denatured DNA template. The complementary DNA strand is synthesized by Klenow enzyme which uses the 3′-OH termini of the random oligonucleotides as primers and a mixture of deoxyribonucleotides containing…

  • ROCHE DIG-High Prime

    MilliporeSigma

    …newly synthesized digoxigenin-labeled DNA after 1 hour, and 2μg after a 20-hour incubation at +37°C. Contents 5x concentrated random primer mix: 1U/ μl Klenow polymerase, labeling grade, 1mM dATP, 1mM dCTP, 1mM dGTP, 0.65mM dTTP, 0.35mM DIG-11-dUTP, alkali labile in 50% (v/v)…

  • …on product ordered): premixed, predispensed tubes containing 30× labeling mix and control DNA. Feinberg and Vogelstein introduced the use of random sequence hexanucleotides to prime DNA synthesis on denatured template DNA at numerous sites along its length. The primer-template complex is a…

  • …effortless setup. Just add your RNA. The two vials included in this kit contain all reagents required for reverse transcription, including random hexamer primer, nucleotides, buffers and enzymes •  Save time with a fast protocol. The optimized master mix significantly reduces reverse…

  • …RNase inhibitor, etc. Application Gene synthesis First-strand synthesis of cDNA from RNA molecules (reverse transcription) RT-PCR Random priming reactions Library construction Probe labeling mRNA 5'-end mapping by primer extension analysis Real time PCR  

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