Proteinase K Solution

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Proteinase K Solution
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  • Proteinase K Solution

    Bioline

    Proteinase K is a highly active serine protease (MW 28,500 Da) isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines. Because the solution is…

  • DNA-pure Yeast Genomic Kit

    …solubilization solution after a 10 minute incubation at 70 C; 4) centrifuge and then isopropanol precipitate DNA from the supernatant; 5) wash pellet and resuspend in TE or water. Advantages:Easy 30 minute protocol High yield of quality, intact yeast genomic DNA. No lytic enzymes or proteinase K

  • ROCHE Proteinase K, recombinant, PCR Grade, Solution from Pichia pastoris

    MilliporeSigma

    …stimulate proteinase K activity, denaturing agents (SDS and urea) can be added. For example, SDS at a final concentration of 2% can increase the activity of proteinase K significantly. Optimization using denaturing agents can increase proteinase activity by as much as sevenfold. Working solution:

  • illustra Blood genomicPrep Mini Flow Kit

    GE Healthcare

    …downstream molecular biological applications. The kit contains spin columns prepacked with a novel silica membrane, lyophilized proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.

  • Proteinase K Solution

    IBI Scientific

    IBI’s Proteinase K Solution is a stable 20mg/ml highly active proteolytic enzyme that is purified from the mold Tritirachium album. The enzyme has two binding sites for Ca++, which lie some distance from the active site and is not directly involved in the catalytic mechanism. The high activity…

  • illustra bacteria genomicPrep Mini Spin Kit

    GE Healthcare

    …method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells. The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution. Contaminants are removed…

  • Proteinase K Solution (20mg/mL)

    Omega Bio-tek

  • Proteinase K from Tritirachium album, buffered aqueous glycerol solution, for…

    MilliporeSigma

    …and its isoelectric point is 8.9. Ca2+ (1-5 mM) is required for its activation. Proteinase K is inhibited by DIFP (diisopropylfluorophosphate) or PMSF (phenylmethylsulfonyl fluoride). Physical form: Solution in 40% glycerol (v/v) containing 10 mM Tris-HCl, pH 7.5, with 1 mM calcium acetate.…

  • ROCHE Proteinase K, recombinant, PCR Grade, Lyophilizate from Pichia pastoris

    MilliporeSigma

    …excess for long incubation periods. Contents Lyophilizate PCR Grade Proteinase K is also available in solution. Application: Proteinase K is one of the most active endopeptidases known. This PCR Grade Proteinase K is particularly suitable for isolating nucleic acids for amplification…

  • Novagen® Proteinase K Solution, 600mAU/mL

    MilliporeSigma

    …The Proteinase K, Lyophilized powder can be prepared as a 20 mg/ml stock solution in water and stored in aliquots at -20°C. The enzyme is also available as a ready-to-use concentrated stock solution (600 mAU/ml) that is convenient for routine use in most applications. 1 mg of Proteinase K is the…

  • illustra blood genomicPrep Mini Spin Kit

    GE Healthcare

    …downstream molecular biological applications. The kit contains spin columns prepacked with a novel silica membrane, lyophilized proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.

  • illustra tissue and cells genomicPrep Mini Spin Kit

    GE Healthcare

    …cells. To minimize the incidence of DNA shearing during extraction, illustra tissue and cells genomicPrep Mini Spin Kit includes a lysis solution with proteinase K to gently release genomic DNA from tissues or cells. The kit can be used to extract DNA from diverse tissue types such as liver, kidney,…

  • Proteinase K

    Minerva Biolabs

    …products. These samples may require an additional Proteinase K treatment to increase the overall DNA yield for highest sensitivity. In combination with Minerva Biolabs´ VenorGeM Sample Preparation Kit 10 µl of the Proteinase K solution is used for each sample after mixing the sample…

  • Proteinase K

    G-Biosciences

    …is 20mM Tris-HCl (pH7.4), 1mM CaCl2, 50% glycerol. Proteinase K is stable for 1 year in this storage buffer, recommended storage is -20°C. LongLife™ Proteinase K LongLife™ Proteinase K is an optimized, 5mg/ml solution that is free from all nuclease contamination. The…

  • Proteinase K

    bioWORLD

    …protein structure. Proteinase K is a highly active protease (MW 28,500 Da) isolated from the fungus Tritirachium album . The enzyme exhibits broad specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA. The solution is tested for the absence…

  • Proteinase K

    IBI Scientific

    Proteinase K is a stable >50 mg/ml (power) highly active proteolytic enzyme that is purified from the mold tritirachium album. The enzyme has two binding sites for Ca++, which lie some distance from the active site and is not directly involved in the catalytic mechanism. The high activity of the…

  • Proteinase K

    Bioline

    …at high temperatures Molecular biology grade - useful for general digestion of proteins Proteinase K is also available as a stabilized stock solution Product Description Proteinase K is a highly active serine protease (MW 28,500 Da) isolated from the fungus Tritirachium album.…

  • Proteinase K, recombinant, PCR Grade

    MilliporeSigma

    proteinase K activity, denaturing agents (SDS and urea) can be added. For example, SDS at a final concentration of 2% can increase the activity of proteinase K significantly. Optimization using denaturing agents can increase proteinase activity by as much as sevenfold. Working solution:

  • Proteinase K Solution

    Omega Bio-tek

    Useful for the inactivation of nucleases and lysis during the isolation of DNA and RNA.

  • SIGMA Proteinase K from Tritirachium album >=500 units/mL, buffered aqueous…

    MilliporeSigma

    Synonym(s): Endopeptidase K MDL Number: MFCD00132129 Storage: 2-8°C

  • ROCHE DNA Isolation Kit for Cells and Tissues

    MilliporeSigma

    …Lysis Buffer • Proteinase K Solution • RNase Solution • Protein Precipitation Solution Preparation Note: Sample material is homogenized in Cellular Lysis Buffer in the presence of a strong anionic detergent and proteinase K. RNA is eliminated by RNase…

  • Quick-RNA FFPE Kits

    Zymo Research Corporation

    …optimized for maximum recovery of both large and small RNA species. Simply deparaffinize tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin column technology. The result is high-quality total RNA…

  • Quick-DNA/RNA FFPE Kits

    Zymo Research Corporation

    …optimized for maximum recovery of DNA and of both large and small RNA species. Simply deparaffinize tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin column technology. The result is high-quality DNA & total…

  • Kininogen, low molecular weight from human plasma, >=95% (SDS-PAGE), lyophilized…

    MilliporeSigma

    …Actions: LMW kininogen is a major extracellular cysteine proteinase inhibitor. Tissue kallikrein releases bradykinin, a potent bronchoconstrictor and vasoactive agent, from both HMW and LMY kininogen. Physical form: Lyophilized from a solution in sodium acetate and sodium chloride.

  • ROCHE Pefabloc® SC

    MilliporeSigma

    …completely inactivate proteinase K during the preparation of chromosomal DNA in agarose plugs. For this purpose, the cells are embedded in agarose and digested with proteinase K to degrade all proteins. Before a specific restriction endonuclease is added, the proteinase K is inactivated by…

  • ROCHE Pefabloc® SC PLUS

    MilliporeSigma

    …completely inactivate proteinase K during the preparation of chromosomal DNA in agarose plugs. For this purpose, the cells are embedded in agarose and digested with proteinase K to degrade all proteins. Before a specific restriction endonuclease is added, the proteinase K is inactivated by…

  • ROCHE Phosphoglucose Isomerase (PGI), from yeast

    MilliporeSigma

    …sulfate solution, pH approximately 6 Preparation Note: Storage conditions (working solution): A solution of PGI in 20 mM Tris, pH 8.1, containing 0.2 mg BSA/ml, is stable approx. 1 week at 2 to 8 °C. Specificity: Phosphoglucose isomerase (PGI) is specific for G6P (K m = 0.7…

  • ROCHE Leupeptin, Ac-Leu-Leu-argininal x 1/2 H2SO4, synthetic

    MilliporeSigma

    …6 months frozen in aliquots at -15 to -25 °C. Specificity: Leupeptin inhibits serine and thiol proteases such as trypsin, plasmin, proteinase K, kallikrein, papain, thrombin, and cathepsin A and B. Not affected are α-, β-, γ- and δ- chymotrypsin, pepsin, cathepsin D,…

  • ROCHE High Pure miRNA Isolation Kit

    MilliporeSigma

    …and more than 50% is recovered with the two-column method. Components: • Tissue Lysis Buffer (for FFPE sections) • Proteinase K, recombinant (for FFPE sections) • Binding Buffer • Binding Enhancer • Wash Buffer • Elution Buffer…

  • Terfenadine

    MilliporeSigma

    …number: MFCD00079622 PubChem Substance: 24277779 Biochem/physiol Actions Terfenadine inhibits HERG (human ether-a-gogo-related gene) K+ channels. It also blocks the delayed rectifier potassium current (IK) of rat isolated ventricular myocytes with IC50 value of 5.96 µM. Non-sedating…

  • Proteinase K 20 mg/ml Solution

    Amresco

    Grade: Biotechnology

  • UREA, 250mL

    Amresco

    …conditions. Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the activity of Proteinase K digestions.

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