IBI’s Proteinase K Solution is a stable 20mg/ml highly active proteolytic enzyme that is purified from the mold Tritirachium album. The enzyme has two binding sites for Ca++, which lie some distance from the active site and is not directly involved in the catalytic mechanism. The high activity of the enzyme solution (0.6 U/μl) means that you can keep the reaction volumes low, as low as 200 mg/mL. It also means that you can expect more complete digestion of your samples.
Store at +4°C.
Tris: 10 mM
Calcium Chloride: 1 mM
Glycerol: 30%
pH: Adjusted to 8.0 with HCl
Molecular Biology Specifications
DNase Assay (Endo and Exo): None Detected
RNase Assay: None Detected
Protease Activity on Gel Substrate: Pass